非水相介质酶法转化制备乙酸3-甲硫基丙醇酯工艺

Lipase-Catalyzed Conversion in Non-aqueous Media for the Preparation of Ethyl-3-methyl-thiopropionate

研究脂肪酶催化合成天然乙酸3-甲硫基丙醇酯的关键因素及条件。结果表明:固定化脂肪酶Novozym 435是无溶剂体系和正己烷体系中最佳催化剂,加酶量、分子筛用量、乙酸加入次数、反应温度、反应时间等因素对乙酸3-甲硫基丙醇酯合成有重要影响。在无溶剂体系体系,加有1.06g 3-甲硫基丙醇、0.6g乙酸的反应中正交试验设计优化的条件为:加酶量50mg、分子筛加入量2g、反应温度42℃、反应总时间30h,乙酸分3次加入,间隔时间为2h,摇床转速为150r/min,乙酸3-甲硫基丙醇酯产率达42.4%。

Ethyl-3-methyl-thiopropionate（EMTP） is an important kind of spice.The key factors and conditions for bio-catalytic synthesis of EMTP in non-aqueous system were studied in the paper.The results indicated that the best catalyst in solvent-free medium or normal hexane was Novozym 435.Catalyst dosage,the amount of molecular sieve addition,the number of acetic acid addition,reaction temperature and reaction time were important factors for EMTP synthesis.The optimal preparation conditions,as determined using orthogonal array design method,were 50 mg of Novozym 435,2 g of molecular sieve,42 ℃ of reaction temperature,30 h of reaction time,three times of acetic acid addition with an interval of 2 h,and 150 r/min of shaking speed.The yield of EMTP reached up to 42.4% in solvent-free medium under the optimal conditions.