柱前衍生高效液相色谱法测定水产品中羟脯氨酸含量

Determination of Hydroxyproline in Fishery Products by Pre-Column Derivatization-High Performance Liquid Chromatography

建立水产品中羟脯氨酸含量的柱前衍生高效液相色谱测定方法。样品经酸水解,丹酰氯衍生,C1 8柱分离,紫外检测器检测。结果表明,羟脯氨酸与其他氨基酸较好分离,在0.5～20μg/mL范围内线性关系良好(r>0.9999),检出限0.5μg/mL,平均回收率80.2%～103%,RSD范围3.2%～5.6%。该方法灵敏、准确,适用于水产品中羟脯氨酸含量的测定。

A high performance liquid chromatographic method was established for the determination of hydroxyproline in fishery products with pre-column derivatization.Samples were hydrolyzed with hydrochloric acid before pre-column derivatization with dansyl chloride.The analyze was separated using a C18 column and detected using a UV detector.Good separation was achieved for hydroxyproline and other amino acids.The linear range was 0.5-20 μg/mL（r 0.9999）.The detection limit was 0.5 μg/mL.The average spike recovery rates were 80.2%-103% with a RSD of 3.2%-5.6%.The method was sensitive,accurate and suitable for the determination of hydroxyproline in fishery products.