北细辛挥发油不同馏分的GC-MS分析研究及急性毒性评价

Studies on the Chemical Constituents of Different Fraction from Asarum Heterotropoides Var. Mandshuricum Volatile Oil By GC-MS and Acute Toxicity of These Fractions

目的:考察北细辛挥发油不同馏分的化学成分及急性毒性,以期为临床安全、合理用药提供依据。方法:采用减压分馏的方法对总挥发油进行分馏,采用GC-MS法分析研究不同馏分的化学成分,采用Bliss法及最大给药量法评价不同馏分急性毒性。结果:北细辛3个馏分挥发油的LD50分别为低沸程油10mL/kg、中沸程油6.5mL/kg、高沸程油15mL/kg。结论:北细辛3个馏分挥发油的急性毒性强弱依次为高沸程(约140℃)、中沸程(80℃～140℃)、低沸程(约80℃)。

Objective：This paper is for the purpose of studying the chemical constituents of different fraction from asarum,to provide a theoratical basis for safe and reasonable use of asarum.Methods：Separating total volatile by vacuum fractionation,analyzing chemical constituents of every fraction by GC-MS,evaluating acute toxicity of every fraction by bliss method.Result：LD50 of low-boiling residue is 10mL/kg,middle-boiling residue is 6.5 mL/kg,high-boiling residue is 15 mL/kg.Conclusion：acute toxicity intensity sequence： high-boiling residue（140℃-）middle-boiling residue（80℃-140℃） low-boiling residue（-80℃）.