营养限制对厌氧序批操作反应器的影响及其恢复重建过程

Effect of nutrients limitation on performance of anaerobic sequencing batch reactor and recovery process

研究了在厌氧条件下以葡萄糖为基质的序批操作反应器(ASBR)中营养物浓度限制对基质吸收和储存的影响及其恢复重建过程。结果表明,营养物限制条件下,发酵细菌表现为过量吸收基质并储存为糖原,形成隐性增殖以维持其细胞的正常结构和代谢功能;而甲烷菌由于无储存能力,其表现为活性逐渐降低。短期营养限制条件下(1个周期),基质中无磷时,储存量增加29%;基质中无氮时,储存量增加90%;基质中既无氮也无磷时,储存量增加26%。长期(31个周期)营养限制(基质中氮磷含量减小50%)下,胞内储存糖原量可高达正常状态下的4.8倍,但甲烷活性减少为正常状态下的17.78%。营养物限制对发酵细菌影响较小,对甲烷菌影响较大。将氮磷浓度恢复正常后,反应器的产甲烷能力恢复较快,出水COD经31周期后恢复正常,而胞内糖原在85周期后恢复正常。

Effect of nutrient limitation in a glucose-fed anaerobic sequencing batch reactor（ASBR） and recovery was investigated.The results show that fermentative bacteria excessively absorbed substrate as glycogen and became recessivity multiplication to hold the normal structure and metabolism under nutrients limitation,and the activity of methanogen without store ability decreased.The storage glycogen increased by 29% under non-phosphorus condition,90% under non-nitrogen condition and 26% without phosphorus and nitrogen.In long term（31 cycles） nutrient limitation,glycogen storage was 4.8 times of normal condition,SMA reduced to 17.78%.Nutrient limitation had less impact on fermentative bacteria and great on methanogen.After the concentration of nitrogen and phosphorus adjusted to normal condition,COD in effluent decreased to normal level after 31cycles,and glycogen storage decreased to normal level after 85 cycles.