摘要
目的研究HIV1/2抗体与P24抗原联合检测对临床诊断的影响。方法自2006年1月-2011年3月分别收集ELISA法检测HIV1/2抗体吸光度(OD值)为临界值及大于等于阳性对照的患者血清33、29份,采用电化学发光法联合检测HIV1/2抗体与P24抗原(HIVCOM),追踪患者免疫印迹HIV确证试验结果,研究HIV1/2抗体与P24抗原联合检测对临床诊断的影响。结果 33例临界值吸光度患者中27例HIV1/2抗体与P24抗原阳性,6例阴性,29例大于等于阳性对照值吸光度的患者中28例阳性,1例阴性,免疫印迹HIV确证试验的检测结果与联合检测HIV1/2抗体及P24抗原结果一致。结论 HIV1/2抗体与P24抗原联合检测较ELISA法能够更早更准确的发现HIV感染,对临床的早期诊断和治疗具有重要的意义及应用价值。
OBJECTIVE To study the effect of combined assay of anti-HIV1,2 and P24 antigen on clinical diagnosis.METHODS A total of 33 and 29 examples of patient serum whose absorbency(OD value) on HIV in ELISA were medi-value or more than positive contral from Jan 2006 to Mar 2011 were separately collected,their anti-HIV1,2 and P24 antigen(HIVCOM) were detected by electrochemistry,tracks for their results of western blot were made,the effects on clinical diagnosis of combined assay of anti-HIV1,2 and P24 antigen were studied.RESULTS There were 27 cases of positive anti-HIV1,2 and P24 antigen,6 negative cases among the 33 patients and 28 positive and 1 negative of the 29 patients by electrochemistry,the results of HIV confirmatory by western blot assay were the same with that of anti-HIV1,2 and P24 antigen by combined assay.CONCLUSION The combined assay of anti-HIV1,2 and P24 antigen can discover the infection of HIV earlier and definitely than ELISA,it has important significance in clinical diagnosis and therapy at the early stage.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2012年第9期1786-1788,共3页
Chinese Journal of Nosocomiology