鸡消化道上皮细胞中乳酸杆菌黏附受体蛋白含量变化

Lactobacillus adhesion receptor protein content of digestive tract epithelial cells in chichen

利用兔抗鸡消化道上皮细胞上乳酸杆菌受体蛋白血清,以琼脂扩散试验、不连续活性-PAGE电泳技术和间接ELISA方法,对1～40日龄、健康和患球虫病鸡消化道不同部位乳酸杆菌黏附受体蛋白含量进行了检测。结果表明,1日龄鸡嗉囊与小肠部位产生乳酸杆菌黏附受体蛋白成分,D450nm值分别为0.236和0.176,4～5日龄乳酸杆菌黏附受体蛋白成分达到稳定,D450nm值分别为0.231和0.166。健康鸡体内嗉囊与小肠部位上皮细胞上乳酸杆菌黏附受体蛋白含量比患球虫病鸡明显增多,D450nm值分别为:嗉囊,0.181和0.164;小肠,0.180和0.161。

Using of antiserum of hare resisting on chicken Lactobacillus adhesion receptor proteins of digestive tract epithelial cells,by agar diffusion experiment,discontinuation activity-PAGE electrophoresis and indirected ELISA technology,detected Lactobacillus adhesion receptor protein contents in crop and intestines of 1-40 days,healthy and coccidiosis chickens.The results suggested that 1 d chicken crop and intestines had Lactobacillus adhesion receptor protein,D450 nm was 0.236 and 0.176 respectively.Stabilization of the protein content was in 4-5 days,D450 nm was 0.231 and 0.166 respectively.Healthy chicken crop and intestines had more lactobacillus adhesion receptor proteins than those of chichens suffering from coccidiosis,D450 nm was 0.181 and 0.164 in crop;0.180 and 0.161 in intestines respectively.