冠心病患者巨噬细胞胆固醇流出的变化

Changes of Cholesterol Efflux of Macrophages in Patients with Coronary Heart Disease

目的研究冠心病患者血液中单核细胞分化为巨噬细胞并荷脂后胆固醇流出的影响机制。方法分离冠心病患者(试验组)和正常人群(对照组)外周静脉血中单核细胞,并用佛波酯诱导为巨噬细胞,收集分离血清,检测各项血脂指标并备用。细胞用[3H]胆固醇处理24 h使其荷脂,然后分为四组:对照组+正常血清、对照组+高脂血清、试验组+正常血清及试验组+高脂血清。分别检测ABCA1、ABCG1表达及胆固醇流出率。结果与对照组比较,试验组单核/巨噬细胞胆固醇流出能力受损,油红O染色显示细胞内脂质蓄积增加,细胞内胆固醇流出减少,但ABCA1和ABCG1的表达改变不明显。与正常血清比较,试验组及对照组中高脂血清明显影响胆固醇流出能力,细胞内脂质蓄积增加,细胞内胆固醇流出减少,但不影响ABCA1和ABCG1的表达。结论冠心病患者巨噬细胞胆固醇流出能力受损,可能与动脉粥样硬化性血管疾病的发生相关。

Aim To study cholesterol efflux of macrophages in patients with coronary heart disease after choles- terol load and its mechanism. Methods Peripheral blood mononuclear cells were separated and induced to macropha- ges with phorbol ester in patients （ test group） and normal individuals （ control group）. Cells incubated with [ 3 H ] choles- terol for 24 h to charge cholesterol and then divided into 4 groups ： control group ＋ normal serum, control group ＋ high-cho- lesterol serum, test group ＋ normal serum and experimental group ＋ high-cholesterol serum. ABCA1, ABCG1 expression, cholesterol efflux rates were detected. Results Compared with control group, monocyte/macrophage cholesterol efflux in experimental group was impaired. Oil Red O stain showed that the accumulation of intracellular lipid increased. The efflux of intracellular cholesterol decreased. But the expressions of ABCA1 and ABCG1 were not obvious. Compared with normal serum, high-cholesterol serum in the test group and control group significantly affected cholesterol efflux capac- ity. The accumulation of intracellular lipid increased. The efflux of intraeellular cholesterol decreased. But the ex- pressions of ABCA1 and ABCG1 were not obvious. Conclusion Macrophage cholesterol efflux in patients with coro- nary heart disease is impaired, which may be related to atherosclerosis-related vascular diseases.