糖尿病大鼠海马血管紧张素转化酶2表达与认知功能的相关性

Association between hippocampal angiotensin-converting enzyme 2 expression and cognitive function in diabetes mellitus rats

目的观察糖尿病大鼠海马血管紧张素转化酶(ACE)2 mRNA和蛋白的表达并探讨其对糖尿病大鼠认知功能的影响。方法 20只雄性SD大鼠随机均分为正常对照组和糖尿病组,建立STZ糖尿病模型,建模成功后11 w用Morris水迷宫测试学习与记忆能力,RT-PCR检测海马ACE2 mRNA的表达,Western印迹检测海马(ACE)2蛋白表达。结果与正常对照组相比,糖尿病组大鼠学习与记忆能力明显减退,其逃避潜伏期时间延长(P<0.05),穿越目标区域次数减少(P<0.05);海马ACE2 mRNA和ACE2蛋白表达明显降低(P<0.05)。结论糖尿病大鼠海马(ACE)2 mRNA和蛋白表达下调与糖尿病大鼠认知功能障碍有关。

Objective To explore the expression of angiotensin-converting enzyme 2（ACE2） in the hippocampus of diabetic rats and its effect on cognitive function.Methods 20 SD male rats were divided into normal and diabetic model groups randomly.Streptozotocin-diabetic rats model were established,11 weeks later,learning and memory behaviors were measured using a spatial version of the Morris water maze test.The mRNA level of ACE2 was examined by RT-PCR.The protein expression of ACE2 was examined by Western blot.Results Compared to the normal group,learning and memory level of diabetic group were decreased transparently（P0.05）,the mRNA and protein levels of ACE2 in hippocampal neuron were decreased（P0.05）.Conclusions The decreased expression of ACE2 mRNA and protein in hippocampus of diabetic rat maybe associate with cognitive dysfunction.