摘要
为研究叶绿体转基因烟草对土壤根际微生物的影响,以aadA叶绿体转基因烟草和对照烟草为实验材料,对苗期、花期、成熟期烟草根际细菌、放线菌及真菌的菌落数进行统计,利用Biolog法分析微生物群落功能多样性.结果表明:在相同生育期,aadA叶绿体转基因烟草与对照烟草相比,根际土壤细菌、放线菌及真菌数量均无显著差异;根际土壤微生物群落的代谢相似,转基因烟草对微生物群落功能的影响不显著.aadA叶绿体转基因烟草没有对土壤微生物数量和功能多样性产生显著的影响.
To study the effects of aadA transplastomic tobacco （TA） on soil microbial communities, the quantity of bacteria, actinomycetes, and fungi was investigated at seedling stage, flowering stage, and senescing stage by plate cultivation of microorganism, and the functional diversity of rhizosphere microorganisms was de- tected by Biolog Ecoplate method. The results showed that there was no significant difference on the popula- tions of bacteria, actinomycetes, and fungi between aadA transplastomic tobacco and non-transgenic tobacco at same growing stage. The Biolog analysis indicated that the metabolism of rhizosphere microbial community were similar, aadA transplastomic tobacco didn＇ t affect the microbial community. In summary, aadA trans- plastomic tobacco had no significant effects on quantity and functional diversity of rhizosphere microorganisms in soil during one year growth.
出处
《哈尔滨工业大学学报》
EI
CAS
CSCD
北大核心
2012年第4期141-144,共4页
Journal of Harbin Institute of Technology
基金
国家自然科学基金资助项目(30970552)
黑龙江省高校科技创新团队研究计划项目(2010TD10)
哈尔滨师范大学科技创新团队研究计划项目(KJTD-2011-2)
