野生保护抚育杠板归药材的品质评价研究

Quality evaluation of wildlife tending Polygonum perfoliatum

目的:采用HPLC测定不同年份贵州省野生护育杠板归药材的指纹图谱,期望能够有效地评价其质量,为杠板归药材的GAP种植提供科学的依据。方法:色谱柱Lichrospher C18(4.6 mm×250 mm,5μm)柱,流速1 mL.min-1,检测波长为340 nm,以乙腈-0.2%醋酸溶液为流动相梯度洗脱。结果:2011年所测产地的杠板归药材比2010年的药材更趋于一致,品质更好;其中大方县的药材在这2年内是最稳定的,贵定县样品GBG-(GD)002和龙里县样品GBG-(LL)003来源地种植的杠板归药材相对其他来源地品质更稳定,基地生产更成熟。结论:建立HPLC指纹图谱的方法来评价各产地杠板归药材的质量与稳定性是可行的,能够从整体上全面而有效地评价杠板归药材的质量,为杠板归药材的GAP种植提供一定的科学依据。

Objective ： To evaluate the quality of Polygonum perfoliatum collected from GAP planting base efficiently so as to provide scientific evidence for its GAP tending plant by HPLC fingerprinting method. Method： Analysis was performed on a reserved- phase column Lichrosher-Cm8 column （4.6 mm×250 mm,5μm）, A linear gradient elution of 0. 2% aqueous acetic acid and acetoni- trile was used for the separation. Result ： Wildlife tending P. perfoliatum was relatively stable. The chromatograms of the samples from 2011 were more accordant than those from 2010. Samples in Dafang were the most stable and had little differences during the two years. The quality of P. pegColiatum planted in the regions of sample GBG-（GD） 002 in Guiding and sample GBG-（LL） 003 in Longli were more stable and better than those from other regions. Conclusion： It is a feasible method that can be used to evaluate the quality of P. perfoliatum for GAP planting.