宣痹凝胶帖膏剂有效成分的透皮行为研究

Transdermal behavior of effective constituents in Xuanbi gel plaster

目的:研究宣痹凝胶贴膏剂中有效成分龙胆苦苷、柚皮苷、原苏木素B的透皮吸收特性。方法:采用Franz扩散池法,以大鼠腹皮为体外模型,用HPLC测定接受液、皮中、膏中残留的龙胆苦苷、柚皮苷、原苏木素B 3种指标成分的含量。结果:宣痹凝胶贴膏剂中龙胆苦苷、柚皮苷、原苏木素B的透皮速率分别为3.47,1.59,2.13μg.cm-2.h-1;24 h的累积透过率分别为25.42%,11.73%,17.78%;在皮中的滞留量分别为0.231,0.593,0.568μg.cm-2,滞留率分别为0.027%,0.227%,0.475%;在膏中的残留量分别为2 179,674,278μg,残留率分别为81.36%,81.92%,73.83%。结论:宣痹凝胶贴膏剂中有效成分的体外透皮行为接近零级过程,皮中滞留率相比透过率和膏中残留率来说非常低。

Objective： To study the transdermal absorbability of gentiopicroside,naringin and protosappanin B contained in Xuanbi gel plaster.Method： The Franz diffusing cells method was adopted for the in vitro model of rat belly skins.Three indexes,gentiopicroside,naringin and protosappanin B,residued in the accept liquid,skins and plaster were determined by HPLC.Result： The penetration rates of gentiopicroside,naringin and protosappanin B were respectively 3.47,1.59,2.13 μg·cm^-2-1.After 24 h,their penetration rates were 25.42%,11.73%,17.78%,respectively.The residual quantities of gentiopicroside,naringin and protosappanin B in skin were 0.231,0.593,0.568 μg·cm^-2ith the retention rates of 0.027%,0.227%,0.475%,respectively.The amount of residue of gentiopicroside,naringin and protosappanin B in plaster were 2 179,674,278 μg,with the retention rates of 81.36%,81.92%,73.83%,respectively.Conclusion： The in vitro transdermal behavior of Xuanbi gel plaster is close to a zero-order process.The residual quantity the retention rate in skins is much lower than the penetration rate and the residual rate in plaster.