孕酮对大鼠脑缺血/再灌注损伤的神经保护作用及其对IP3含量的影响

Neuroprotection of progesterone against cerebral ischemia-reperfusion injury on rats and the effect on IP3 content

目的探讨孕酮对大鼠局部脑缺血/再灌注损伤的神经保护作用及机制。方法将大鼠随机等分为假手术组、正常对照组、孕酮组、溶剂(DMSO)组,采用线栓法制作大鼠局部脑缺血再灌注模型,TTC染色和ELISA法分别测定大鼠大脑梗死面积和梗死侧IP3的含量。结果孕酮(8 mg/kg)治疗后大鼠脑梗死面积比例明显小于溶剂组(P<0.05);溶剂组脑梗死侧的IP3含量显著低于假手术组和正常对照组,孕酮组脑梗死侧的IP3含量高于溶剂组(P<0.05)。结论孕酮对大鼠局部脑缺血再灌注损伤有明显的神经保护作用,可能与通过恢复大鼠梗死侧大脑组织IP3有关。

Objective To investigate the neuroprotective effects and mechanism of progesterone on ischemia-reperfusion injury.Methods The rats were divided randomly into sham operation group,normal control group,progesterone（PROG） treament group and dimethl sulfoxide（DMSO） group.The models of cerebral ischemia-reperfusion were made by occlusion of middle cerebral artery by an intraluminal filament method.TTC staining and ELISA methods were used to analyze the proportion of infarction size and IP3 content in region around infarction area.Results The proportion of infarction size in rats with PROG（8 mg / kg） treated was smaller than that in the DMSO group;The IP3 content of DMSO group were lower than that in the sham operation group and normal control group significantly（P〈0.05）,and the IP3 content of PROG treatment group was higher than that in DMSO group significantly（P〈0.05）.Conclusion The results suggest that PROG has significant neuroprotective effects,which may be related with restoring the IP3 content in region around infarction area of rats.