临床标本中沙眼衣原体噬菌体Vp1基因及血清Vp1抗体的检测

Detection of Chlamydia trachomatis phage Vpl gene in clinical swab specimens as well as anti-Vpl anti- bodies in serum specimens

目的检测临床标本中沙眼衣原体噬菌体Vp1基因及血清Vp1抗体。方法收集天津性传播疾病研究所就诊患者的分泌物拭子和血清；用PCR法筛查分泌物标本中的Vp1基因；以Vp1蛋白作为抗原通过ELISA法及Western印迹法检测血清中针对Vp1抗体的存在；对PCR法结果阳性的分泌物进行细胞培养，然后免疫荧光法进行检测Vp1。结果共筛查出36例拭子的PCR扩增产物在目的片段位置出现明显条带，及23例Vp1抗体阳性的血清。通过细胞培养及免疫荧光法，利用制备的单抗检测沙眼衣原体临床标本，尚未发现阳性标本。结论从临床拭子及血清中成功筛查出沙眼衣原体噬菌体Vp1基因和Vp1抗体。

Objective To detect Chlamydia traehomatis phage Vpl gene in clinical swab specimens and anti-Vpl antibodies in serum specimens. Methods Cervical and urethral swab as well as serum specimens were collected from attendees to the sexually transmitted disease （STD） clinic in the Tianjin Institute of STD, during March 2008 to March 2011. PCR was conducted to detect chlamydial phage Vpl gene in swab samples, enzyme linked immunosorbent assay （ELISA） and Western blot to detect anti-Vpl antibody in sera. The swab specimens positive for Vpl gene were subjected to cell culture followed by the detection of Vpl protein with an immunofluorescence-based method. Results Totally, 36 out of 1542 swab specimens turned out to be positive for Vpl gene, and 23 out of 453 serum specimens for anti-Vpl antibody. No positive results were obtained in the Vpl gene-positive swab specimens by cell culture and immunofluorescence-based assay. Conclusion The Vpl gene of Chlamydial trachomatis phage and anti-Vpl antibody are successfully detected from clinical swab and serum specimens respectively.