摘要
目的探讨小鼠白介素2重组质粒对沙眼衣原体E型DNA疫苗细胞免疫效果的影响。方法以BALB/c小鼠为实验动物,采用肌内接种免疫的方式,将小鼠分为接种空质粒的阴性对照组、DNA疫苗免疫组、DNA疫苗联合小鼠白介素2重组质粒免疫组和接种灭活沙眼衣原体E型原体的阳性对照组,观察小鼠后足垫局部的肿胀情况、脾淋巴细胞增殖试验、血清IL-4和IFN-γ水平和同型沙眼衣原体生殖道攻击后局部的衣原体培养情况。结果4组实验动物的脾淋巴细胞增殖指数分别为1.37±0.21、2.524-0.30、3.644-0.41、3.774-0.34,联合免疫组和阳性对照组比较差异无统计学意义,与另2组比较,差异有统计学意义。4组的血清IL4分别为(25.37±18.93)、(24.75±8.49)、(21.74±6.43)、(38.49±12.24)pg/ml,灭活原体组高于其他各组;4个组的血清IFN-γ分别为(310.8±160.7)pg/ml、(601.3±357.9)pg/ml、(1923.3±518.1)pg/ml、(2712.5±887.2)pg/ml,联合免疫组和阳性对照组相当,与另两组比较,差异有统计学意义。沙眼衣原体E型原体攻击后,空质粒组小鼠生殖道局部能够培养出衣原体;另3个组衣原体培养阴性。结论白介素2基因佐剂能够增强沙眼衣原体E型DNA疫苗的细胞免疫效应,且以Th1型反应为主。
Objective To study the effect of a recombinant plasmid encoding mouse interleukin 2 (rolL-2) on the immunogenicity of DNA vaccine against Chlamydia traehomatis (Ct) serovar E. Methods BALB/c mice were divided into 4 groups to be intramuscularly inoculated with blank plasmid (negative control group), DNA vaccine against Ct serovar E (DNA vaccine group), DNA vaccine against Ct serovar E and a recombinant plasmid containing raiL-2 (combination group), and inactivated Ct serovar E elementary bodies (positive control group), respectively. The immunological effects were evaluated by posterior foot pad thickness, proliferation level of spleen lymphocytes, serum level of IL-4 and interferon (IFN)-γin mice, and the capability to clear Ct genital tract infection. Results The proliferation index of spleen lymphocytes in the combination group and positive control group was similar (3.64 ± 0.41 vs. 3.77 ±0.34), but was significantly different from that in the blank control group and DNA vaccine group (1.37± 0.21 and 2.52 ± 0.30). The serum level of IL-4 was (38.49 ± 12.24) pg/ml in the positive control group, significantly higher than in the negative control group, DNA vaccine group and combination group ((25.37 ±18.93), (24.75 ± 8.49), (21.74± 6.43) pg/ml,respectively). With respect to the serum level of IFN-γ, the combination group and positive control group were similar (1923.3 ± 518.1 ) pg/ml vs. (2712.5 ±887.2) pg/ml), but were significantly different from the negative control group and vaccine group ( (310.8 ± 160.7) pg/ml and (601.3 ± 357.9) pg/ml). Six days after Ct challenge, the exfoliated cells from genital tract were positive for Ct culture in the negative control group, but negative in the other 3 groups. Conclusion IL-2 genetic adjuvant can enhance the immune response, especially Thl type response, induced by the DNA vaccine against Ct serovar E.
出处
《中华皮肤科杂志》
CAS
CSCD
北大核心
2012年第5期322-324,共3页
Chinese Journal of Dermatology
基金
国家自然科学基金(30691879),天津医科大学基金(2008KY23)