NaCl胁迫对结荚期菜用大豆叶片游离态多胺水平的影响

Effects of NaCl stress on free polyamine level in vegetable-use soybean leaves at podding stage

采用蛭石栽培,在100mmol·L-1NaCl胁迫下,对耐盐性不同的2个菜用大豆[Glycinemax(L.)Merr.]品种结荚期干物质积累、单株产量及叶片游离态多胺(PAs)水平的变化进行了研究。结果表明:NaCl胁迫显著降低了菜用大豆植株干重及单株产量,但耐盐品种"绿领特早"的降幅低于盐敏感品种"理想高产95-1";与"理想高产95-1"相比,"绿领特早"叶片在整个NaCl胁迫期间均维持了相对较低的H2O2含量、游离态腐胺(Put)含量及较高的游离态亚精胺(Spd)含量,在胁迫6～15d期间维持了相对较高的游离态精胺(Spm)含量、(Spd+Spm)/Put值及较低的Put/PAs值。说明耐盐品种"绿领特早"叶片具有较强的由游离态Put向游离态Spd和Spm转化的能力,维持了较低的游离态Put含量和较高的游离态Spd及Spm含量,进而抑制了活性氧过量积累。

Taking two vegetable-use soybean ／[Glycine max （L.） Merr.）／] cultivars with different salt tolerance as test materials, a vermiculite culture experiment was conducted to study their dry matter accumulation, fresh pod yield per plant, and leaf free polyamine level at podding stage under the stress of 100 mmol NaCl·L-1. For the two cultivars, their dry mass and fresh pod yield per plant were decreased significantly under the NaCl stress, and the decrements for salt-resistant cultivar ＂Lülingtezao＂ were lower than those for salt-susceptible cultivar ＂Lixianggaochan 95-1＂. As compared with ＂Lixianggaochan 95-1＂, ＂Lülingtezao＂ had relatively lower leaf H2O2 and free putrescine （Put） contents and relatively higher leaf free spermidine （Spd） content during the period of NaCl stress, and maintained relatively higher leaf free spermine （Spm） content and （Spd＋Spm）/Put ratio and relatively lower leaf Put/PAs ratio in the 6-15 days of the NaCl stress. All the results suggested that ＂Lülingtezao＂ leaves possessed stronger capability for the transformation of free Put to free Spd and free Spm, and maintained lower free Put content but higher free Spd and free Spm contents, which effectively inhibited the excessive accumulation of reactive oxygen.