摘要
简单重复序列间区(inter-simple sequence repeat,ISSR)标记技术近年来已成为DNA分子标记技术中的一个热点,并在遗传学研究中发挥着重要的作用。然而,相比较其在植物研究中的应用,在动物研究中还处于起步阶段。本文就ISSR分子标记技术的原理和方法要点进行简述,归纳了其在动物遗传多样性、资源鉴定、亲缘关系等遗传结构相关领域中的应用情况,并对其应用前景进行了展望,旨在为ISSR分子标记技术在动物相关研究领域中的推广应用提供参考。
Inter-simple sequence repeat (ISSR) marker technology has become a hotspot in DNA molecular technology in recent years, and plays an important role in genetic research. However, this technology is still at an initial stage in animal research, compared with its applications in plant research. This paper introduced the principles and main methods of ISSR marker technology, and summarized its applications in analyzing the genetic structure of animals, such as genetic diversity, resources identification, and genetic relationship, etc. The application prospects of this technology were also discussed, aimed to provide reference for the further applications of this technology in animal research.
出处
《生态学杂志》
CAS
CSCD
北大核心
2012年第5期1319-1326,共8页
Chinese Journal of Ecology
基金
浙江省研究生创新科研项目(YK2009038)资助
关键词
ISSR
遗传多样性
资源鉴定
亲缘关系
ISSR
genetic diversity
resources identification
genetic relationship