强直性脊柱炎患者外周血T细胞受体β链可变区基因谱系多态性的初步研究

Study on the T cells of T cell receptors BV complementarity determining region 3 lineage polymorphism with peripheral blood in ankylosing spondylitis patients

目的探讨强直性脊柱炎（AS）患者外周血T细胞受体B链可变区互补决定区3（TCRBVCDR3）谱系多态性，为AS免疫发病机制研究提供实验依据。方法采用反转录-聚合酶链反应（RT—PCR）扩增AS患者外周血单个核细胞（PBMC）中T细胞TCRBV的26个亚家族CDR3，经免疫扫描谱型技术分析TCRBVCDR3的谱系多态性情况。结果20例活动期AS患者TCRBVCDR3扫描谱型均出现非正态的异常峰型，包括单峰、寡峰／寡峰趋势、偏峰和不规则异常峰型。其中有18例患者部分亚家族出现寡克隆／寡克隆趋势增生，有1例患者BV16和BV18的2个亚家族出现单克隆增生。5名健康对照PBMCTCRBVCDR3谱型绝大多数呈高斯分布。结论AS患者外周血TCRBVCDR3谱系具有显著多态性和谱系漂移特点，进一步表明T细胞在AS免疫发病机制中扮演重要角色。单，寡克隆增生的T细胞有可能是AS发病中的自身反应性T细胞。

Objective To study the T cells lineage polymorphism of TCR BV CDR3 in the peripheral blood of ankylosing spondylitis （AS） patients, in order to provide experimental basis for the immunological patho-genesis study of AS. Methods Twenty-six subfamilies of CDR3 T cells of TCR BV in the PBMC of AS patients were amplified by RT-PCR method, then TCR BV CDR3 lineages polymorphism were analyzed by immunization scanning spectrum. Results TCR BV CDR3 scanning spectrum of 20 active AS patients showed abnormal distribution peak, including monoclonal, oligoclonal/oligoclonal trend, skewing peak and irregular abnormal peak. Among them, some subfamilies of 18 patients showed oligoclonal/oligoclonal trend expansion, BVI6 and BV18 two subfamilies of one case showed monoclonal expansion. Most spectral type of PBMC TCR BV CDR3 in five normal controls showed Gauss distribution. Conclusion TCR BV CDR3 lineage have significant characteristic polymorphism and spectrum drift characteristics in the peripheral blood of AS patients, which further indicate that T ceils has plaied an important role in the immunological pathogenesis of AS. Monoclonal/oligoclonal expansion of T ceils may be autoreactive T cells in nature and they may be involved in the pathogenesis of AS.