氯胺酮抑制昆明小鼠扣带后回Fos表达

Ketamine administration inhibits c-Fos expression in mice retrosplenial cortex

目的探讨慢性氯胺酮使用对扣带后回e-Fos与磷酸化的转录因子环磷酸腺苷反应元件结合蛋白（cAMP responsive element bindingprotein，CREB）的影响。方法成年昆明小鼠，采用随机数字表法分组：腹腔注射50、100、200mg／kg组以及生理盐水组，5d给药1次，共注射6次后4％多聚甲醛灌注固定、取脑、30um冰冻切片，应用免疫组化法检测c-Fos和磷酸化cAMP反应元件结合蛋白（phosphorylated cAMP responsiveelement binding protein，pCREB）的表达。结果①扣带后川和压部后皮质有一定的c—Fos与pCREB基础；②氯胺酮慢性使用扣带后回神经元的c—Fos表达减少，50mg／kg组、100mg／kg组、200mg／kg组分别下降到生理盐水组的80％、43％和37％。而pCREB的表达增加，50mg／kg组、100mg／kg组、200mg／kg组分别增加到生理盐水组的180％、195％和170％。结论慢性氯胺酮使用抑制昆明小鼠扣带后回神经元c-Fos表达，其抑制可能与pCREB表达增加有关。

Objective To investigate the effects of ketamine administration on expression of c-Fos and pCREB in Kunming mice retrosplenial cortex. Methods The adult male Kumning mice were randomly allocated into four groups,control, K1, K2, K3 and groups, in which normal saline and different doses of ketamine including 50, 100, 200 mg/kg were intraperitoneally injected at 5-clay interval for successively 6 times, respectively. The expression of c-Fos and pCREB in retrosplenial cortex were detected via immunohistoehemistry method. Results （ There was a basal expression of c-Fos and pCREB in retrosplenial cortex. （ Chronic ketamine administration dose-dependently downregulated the expression of c-Fos in retrosplenial cortex. Compared to saline control group, ketamine with different doses decreased the c-Fos expression to 80%, 43% and 37%, and increased the expression of pCREB to 180%, 195% and 170%. Conclusions Chronic ketamine injection inhibits the expression of c-Fos expressions in retrosplenial cortex, which may be related to the upregulation of pCREB expression.