急性肺损伤大鼠肺组织中颗粒溶素的表达

Increased Expression of Granulysin in Lung Tissue of Rats with Acute Lung Injury

目的在内毒素(LPS)诱导的急性肺损伤(ALI)大鼠模型中,观察颗粒溶素在不同时期的表达,探讨其在革兰阴性菌感染所致的ALI的细胞免疫中的地位和作用。方法采用36只健康Wistar大鼠,随机分为对照组(NS组)和实验组(LPS组),每组18只。LPS组腹腔注射LPS 4 mg/kg制造ALI模型,NS组注射等量生理盐水。注药后6、18及30 h取材,行肺组织湿/干重比(W/D)分析,观察肺组织病理改变,以及免疫组织化学法检测肺组织颗粒溶素的表达。结果 ALI大鼠肺组织W/D比值显著高于NS组(P<0.05);病理显示LPS组肺组织受损,出血、渗出明显,达到ALI诊断标准;LPS组各时点肺组织颗粒溶素表达较NS组有不同程度增加。结论颗粒溶素可能参与ALI的炎症过程,有助于病原体的清除,在防治感染性因素所致ALI中有可能发挥一定的作用。

Objective To investigate the expression of granulysin（GNLY） in lung of rats with acute lung injury（ALI） stimulated with lipopolysaccharide（LPS）.Methods Thirty-six healthy adult Wistar rats were randomly divided into a normal control group and a LPS group,with 18 rats in each group.LPS（4 mg/kg） was given intraperitoneally in the LPS group to induce ALI.The same amount of normal saline was given in the control group.The rats were randomly assigned to three subgroups（n = 6） to be sacrificed respectively at 6,18,and 30 hours after intraperitoneal injection.Wet/dry lung weight ratio（W/D） and pathological changes of the lung were observed.The expression of GNLY in lung tissue was assayed by immunohistochemistry.Results In the LPS group,the W/D ratio was higher than that of the control group at each time point（P 0.05） and there were a large number of inflammatory cells infiltration and edema in interstitial spaces which suggested ALI.Compared with the control group,the expression of GNLY in the LPS group was significantly increased at all time points（P 0.05）.Conclusion GNLY may participate in ALI inflammatory process,which might play a role in preventing infection induced ALI.