p53基因敲除小鼠的饲养繁殖及鉴定

Breeding Reproducing and Identifying for p53 Gene Knockout Mice

目的为了繁育和鉴定p53基因敲除小鼠,将引进的杂合子小鼠进行饲养繁殖,杂合子用于继续保种。方法对其幼鼠剪尾提取基因组DNA,采用PCR方法进行基因型鉴定。结果对引进小鼠已成功饲养和繁殖,并得到纯合基因缺失型小鼠。结论正确的饲养、繁殖及基因鉴定方法对于基因敲除小鼠的获得和保种具有重要的意义。

Objective To breed and identify p53 gene knockout mice,Heterozygote mice were bred and reproduced.Methods Genome DNA extracted from the mice＇s tails were subjected to PCR test for genotype identification.Results Heterozygous were used to acquire baby mice for Protection species.Conclusion The breeding and reproducing were successful and Homozygous genotype mice were acquired.Appropriate methods of feeding,breeding and identifying are important for obtaining gene knockout mice and protecting species.