EchinocandinB微生物转化工艺优化

Optimization of Microbial Transformation Process of Echinocandin B

犹他游动放线菌产生的酰基水解酶可以将echinocandin B(ECB)水解脱去酰基侧链得到ECB母核,用于化学合成抗真菌药阿尼芬净。本研究考察并优化了犹他游动放线菌产酶的发酵培养基组分和ECB转化反应体系的有机溶剂、底物浓度,建立了ECB的微生物转化工艺。结果表明,在含麦芽糖30 g/L和麦芽浸粉20 g/L的发酵培养基中,犹他游动放线菌发酵培养72 h后,加入终浓度为2.55 g/L的ECB和7.5%甲醇,转化18 h,ECB的转化率为97.8%。进一步在5 L发酵罐中进行发酵培养和微生物转化,结果表明该微生物转化工艺可用于制备ECB母核。

The deacylase from Actinoplanes utahensis could cleave the linoleoyl side chain of echinocandin B（ECB） to produce its nucleus which was used to synthesize the anti-fungal agent anidulafungin.After optimization of the culture medium for deacylase production,suitable solvent and ECB concentration of reaction mixture,a microbial transformation process of ECB was established.The results showed that when the A.utahensis was cultured in the medium containing 30 g/L maltose and 20 g/L malt extract for 72 h,the final concentration of ECB and methanol of 2.55 g/L and 7.5%,and the convertion period of 18 h,the convertion rate of ECB could achieve to 97.8%.Furthermore,fermentation cultivation and biotransformation was conducted in a 5 L fermentor,the results indicated that the enzymatic process could be used for the preparation of ECB nucleus.