石蝉草中草胡椒素B的HPLC法测定

Determination of Peperomin B in Peperomia dindygulensis Miq. by HPLC

建立了高效液相色谱法测定石蝉草中的草胡椒素B。使用C18色谱柱,以乙腈-水(55∶45)为流动相,检测波长210 nm。草胡椒素B浓度在1.2～60 g/ml范围内线性关系良好,回收率为99.2%,RSD为1.0%。

An HPLC method was established for the determination of peperomin B in Peperomia dindygulensis Miq..A C18 column was used with the mobile phase of acetonitrile-water（55∶45） at the detection wavelength of 210 nm.The calibration curve of peperomin B was linear in the range of 1.2-60 μg/ml.The average recovery was 99.2%,with RSD of 1.0%.