摘要
建立了柱前衍生化HPLC-荧光法测定5-氨基酮戊酸。以荧光胺为荧光衍生化试剂,使用C18柱,以乙腈-0.1%三氟乙酸溶液(30︰70)为流动相,激发波长和发射波长为398和480 nm。5-氨基酮戊酸在0.047 5～47.5 g/ml浓度范围内线性关系良好。回收率为99.2%,RSD为1.46%。
A pre-column derivatization HPLC-FD method was established for the determination of 5-aminolevulinic acid.Fluorescamine was used as the derivatizing agent.A C18 column was used with the mobile phase of acetonitrile-0.1% trifluoroacetic acid solution（30︰70）.The excitation and emission wavelengths were 398 and 480 nm.The calibration curve of 5-aminolevulinic acid was linear in the concentration range of 0.047 5-47.5 μg/ml.The average recovery was 99.2%,with RSD of 1.46%.
出处
《中国医药工业杂志》
CAS
CSCD
北大核心
2012年第5期377-380,共4页
Chinese Journal of Pharmaceuticals
基金
上海市自然科学基金项目(11ZR1432800)
关键词
5-氨基酮戊酸
荧光胺
高效液相色谱
柱前衍生化
荧光检测
测定
5-aminolevulinic acid
fluorescamine
HPLC
pre-column derivatization
fluorescence detection
determination
