复方灵芝乳膏对兔耳轻(Ⅱ)度冻伤治疗作用的初步研究

Research on the Therapeutic Effect of Compound Lingzhi Cream for Mild Frostbite on Rabbit Ears

目的考察复方灵芝乳膏对家兔耳轻度冻伤模型的治疗作用。方法将家兔耳浸入-24.5℃酒精浴冷冻槽中20 s,复制家兔耳轻度冻伤模型,涂抹不同浓度的复方灵芝乳膏,观察兔耳表观变化、肿胀程度、存活面积和冻区病理学检查等指标,考察复方灵芝乳膏家兔耳轻度冻伤模型的治疗作用。结果复方灵芝乳膏2号对家兔耳轻度冻伤有一定的治疗作用,但其治疗作用不如复方灵芝乳膏3号(每1 g乳膏含灵芝生药0.5 g、王不留行0.25 g)和4号(每1 g乳膏含灵芝生药1 g、王不留行0.5 g)明显;复方灵芝乳膏3号、4号对家兔耳轻度冻伤有明显的治疗作用。结论复方灵芝乳膏对兔耳轻(Ⅱ)度冻伤治疗作用的初步研究表明,复方灵芝乳膏3号和4号具有深入研究的价值。

OBJECTIVE To explore the therapeutic effect of compound Lingzhi cream for mild frostbite on rabbit ears. METH- ODS Rabbit ears were immersed into alcoholic freezing chamber at -24. 5 ℃to cause mild frostbite, then the therapeutic effect of compound Lingzhi cream of different formula was explored after smearing the cream on the injured site, using appearenee change, swelling and distension degree, survival area and pathological change of the ears as the observation index. RESULTS COmpound Lingzhi cream formula 2 was effective on mild frostbite of rabbit ears to some extend, but the effect was not as obvious as those of com- pound Lingzhi cream formula 3 and 4. CONCLUSION The effective dosage of compound Lingzhi cream on mild frostbite of rabbit ears is 0. 5 g crude Lucid Ganoderma and 0. 25 g crude Cowherb Seed in 1 g cream. The optimal dosage is 1 g cream containing 1 g crude Lucid Ganoderma and 0. 5 g crude Cowherb Seed. The therapeutic effect would not further increase when increasing the drug dosage.