天麻素对海马内Aβ_(1-40)注射阿尔茨海默病模型大鼠学习记忆的改善作用

Improving effects of gastrodin on learning and memory in model rats with Alzheimer disease induced by intra-hippocampal Aβ_(1-40) injection

目的:观察天麻素对海马内β-淀粉样蛋白1-40(Aβ1-40)注射阿尔茨海默病(Alzheimer dis-ease,AD)模型大鼠空间学习记忆能力的影响。方法:双侧海马内注射Aβ1-40建立AD大鼠模型,并设注射生理盐水的假手术组(n=7)和不作处理的正常组(n=10);AD模型大鼠分设天麻素组、石杉碱甲组和模型组(各n=8),分别给予药物或生理盐水灌胃4周(q.d.)后,用Morris水迷宫进行学习记忆能力检测。结果:与正常组或假手术组相比,模型组大鼠在Morris水迷宫的定位航行试验中逃避潜伏期明显延长(P<0.01或P<0.05)。与模型组比较,天麻素组大鼠的逃避潜伏期明显缩短(P<0.05),且空间探索试验中在原平台所在象限停留时间明显延长(P<0.05)。结论:天麻素对海马内Aβ1-40注射AD模型大鼠的学习记忆损害有一定的改善作用。

AIM： To observe the effects of gastrodin treatment on learning and memory of model rats with Alzheimer disease（AD） induced by intra-hippocampal Aβ1-40 injection.METHODS： The AD rat model was established by injection of Aβ1-40 into bilateral hippocampus,rats in sham group（n=7） was injected with saline,and normal group（n=10） without any treatment.The AD model rats in gastrodin group,huperzine A group and model group（n=8 each group）,were administered by oral gavage of drugs or saline,respectively,for 4 weeks（q.d.）.Then Morris water maze was used to examine the changes of learning and memory abilities in rats.RESULTS：The test of Morris water maze showed that the escape latency of place navigation trials in model group was significantly longer than normal or sham groups（P0.01 or P0.05）.In comparison with model group,the escape latency in gastrodin group was significantly shorter（P0.05）,but the time in presumable plate quadrant of spatial probe trial was longer（P0.05）.CONCLUSION： Gastrodin may have improving effect on the abilities of learning and memory of the model rats with AD induced by intra-hippocampal Aβ1-40 injection.