骨肉瘤组织中EphA2和CyclinD1的表达及临床意义

Expression and clinical significance of EphA2 and CyclinD1 in osteosarcoma tissue

目的探讨CyclinD1和EphA2在骨肉瘤组织中的表达和临床意义。方法应用免疫组织化学sP法分别对50例骨肉瘤组织、20例骨软骨瘤组织及20例正常骨组织中EphA2和CyclinD1蛋白的表达进行检测并对结果进行统计学分析。结果正常骨组织、骨软骨瘤组织CyclinD1和EphA2蛋白阳性表达率为0（0／20），骨肉瘤组织中CyclinD1和EphA2蛋白阳性表达率分别为70．00％（35／50）和78．00％（39／50），差异均有统计学意义（P〈0．05）。I级、Ⅱ级和Ⅲ级骨肉瘤组织中EphA2和CyclinD1蛋白阳性表达率在不同临床分期之间两两相比差异有统计学意义（P〈0．05）。伴有肺转移骨肉瘤组织中EphA2和CyclinD1蛋白阳性表达率与无肺转移骨肉瘤组织表达率相比，差异有统计学意义（P〈0．05）。EphA2和CyclinD1蛋白在骨肉瘤组织中的表达，经统计学分析，呈明显正相关（P〈0．01）。结论EphA2和CyclinD1蛋白表达与骨肉瘤的演进有关，EphA2和CyclinD1蛋白在骨肉瘤组织中的表达有相关性。同时，二者可能在骨肉瘤的发生、发展及浸润、转移过程中起协同作用。

Objective To study the expression and clinical significance of EphA2 and CyclinD1 in osteosarcoma tissue. Methods The expressions of EphA2 and CyclinD1 in 50 cases of osteosarcoma tis- sues, 20 cases of osteochondroma tissues and 20 cases of normal bone tissues were deteted by immunohisto- chemistry SP method, and the results were analyzed statistically. Results The positive expression rates of EphA2 and CyclinD1 proteins in normal bone and osteochondroma tissues were 0（0/20）; The positive ex- pression rates of EphA2 and CyclinD1 proteins in osteosarcoma tissues were 78. 00% （39/50）and 70% （35/ 50）, respectively. There were significant differences （ P 〈 0. 05 ）. The positive expression rates of EphA2 and CyclinD1 proteins in osteosarcoma tissues with grade III were higher than those in grade Iand grade ]1. There were significant differences between different clinical stages （P 〈 0. 05 ）. There were significant differences in the positive expression rates of EphA2 and CyclinD1 proteins in osteosarcoma tissues with and without metastasis （P 〈 0. 05）. There was positive correlation of the EphA2 expression with CyclinD1 ex- pression in osteosarcoma tissues （P 〈 0. 01 ）. Conclusions The expressions of EphA2 and CyclinD1 pro- teins are related with progression of osteosarcoma. There is positive correlation between the expressions of EphA2 and CyclinD1 proteins in osteosarcoma tissues. EphA2 and CyclinD1 protein can play synergistic effect on the process of occurrence, development and infiltration, metastasis of osteosarcoma.