黄麻种质资源遗传多样性的SRAP分析

Analysis of Genetic Diversity of Jute(Corchorus L.) Germplasm Revealed by SRAP

为明确黄麻(Corchorus L.)种质资源的遗传多样性,采用SRAP分子标记方法,对来自13个国家的两个黄麻栽培种及野生种共96份黄麻种质资源进行了分析。研究结果如下:(1)供试黄麻种质资源之间的遗传距离在0.0169至0.9667之间,变幅较大,其中近缘野生种与其他种质的遗传距离最大,基本在0.8以上;圆果栽培黄麻与其他种质的遗传距离最小,平均<0.5。(2)当在聚类图上遗传距离为0.53处划切割线L1时,96份黄麻种质资源被分为两个大类群(Ⅰ、Ⅱ)、一个小类群(Ⅲ)和5个独立个类。(3)当在遗传距离为0.33处作切割线L2时,各大类群被分为不同的亚类群,并表现出按地域聚类的趋势。(4)供试黄麻种质资源基于SRAP分子标记的聚类与形态学上的表现并不完全一致。

To clarify the genetic relationship of 96 jute（Corchorus L.） accessions including 6 species from 13 countries,sequence-related amplified polymorphism（SRAP） molecular mar-kers were used in the present study.The results showed that：（1） Genetic distance of 96 jute germplasms exhibited a large variation from 0.0169 to 0.9667,among which the relative wild species had the largest genetic distance with other germplasms,while the C.capsularis cultivars had the least genetic distance with others.（2） All 96 germplasms were classified into 3 groups and 5 unique types at the level of L1=0.53.（3） Each group was classified into different sub-groups at the level of L2=0.33,which showed classification according to their origins.（4） The classification of jute germplasms based on SRAP markers did not necessarily show a relationship with morphological performance.