单抗捕获ELISA检测PRRSV抗体的方法的建立

Establishment of A Capture ELISA with McAb for Detection of Antibodies Against PRRSV in Swine Sera

以国内刚刚研制出的PRRSV单克隆抗体为基础，建立了检测PPRS病病毒抗体的单克隆抗体捕获抗原的ELISA法。其单抗包被浓度为1．16μg／mL，粗提病毒反应浓度为16.50μg／mL，血清稀释度为1：40。经与中和试验以及进口ELISA试剂盒比较，证明该法敏感性高，特异性良好．是一种有应用前景的方法。

With monoclonal antibodies (McAb)against PRRSV,a capture ELISA for detecting serum antibodies against PRRSV was developed for the first time. The concentration of coating McAb was 1. 16μg/ml,the reaction concentration of the roughly extracted PRRSV was 16.50μg/ml,and the optimal dilutionrate 0f serum was 1: 40. Compared with the SNT and the ELISA kit from America(Herd Chek,IDEXX),theresult shows that this methord is more sensitive than the others and has a good specificity. It suggests thatthis method will be useful for detecting antibodies against PRRSV in swine sera.