生物强化去除吡啶的特性及微生物种群动态变化分析

Bioaugmented Removal of Pyridine and the Microbial Community Dynamic Analysis

在接种活性污泥的序批式反应器中投加固定化吡啶降解菌Paracoccus sp.KT-5,强化吡啶的生物降解,并与未投加固定化微生物的反应器进行对照,通过末端限制性片段长度多态性(T-RFLP)分析手段,探讨了运行过程中生物强化与未强化反应器中微生物群落结构的动态变化,并探讨了生物强化反应器的去除效果.结果表明,投加固定化吡啶降解菌可以加速反应器的启动.当吡啶初始浓度为195.6～586.8 mg.L-1,随着反应器的运行,投加固定化菌株的强化作用并不明显;但当吡啶初始浓度为782.4～2 934 mg.L-1,投加固定化菌株显示出优势.T-RFLP分析结果表明,投加的固定化菌株KT-5作为优势菌始终存在于反应器的固定化生物相和悬浮生物相中.

The bioaugmented removal of pyridine was investigated through introducing immobilized Paracoccus sp.strain KT-5 capable of degrading pyridine into the lab-scale sequencing batch reactor(SBR) inoculated with activated sludge.The terminal restriction fragment length polymorphisms(T-RFLP) was used to analyzed the microbial community dynamics of two reactors during the whole operation process.The experimental results indicated that the introduction of immobilized strain KT-5 into the SBR could speed up the start-up of reactor,compared to the non-bioaugmented SBR.When the initial concentration of pyridine varied from 195.6 mg·L-1 to 586.8 mg·L-1,the bioaugmented effect was not significant;however,when the initial concentration of pyridine was 782.4-2 934 mg·L-1,the bioaugmentation role in pyridine degradation was obvious.The analysis of T-RFLP indicated that the introduced immobilized strain KT-5,as a dominant strain,always existed in both free and immobilized biomass of the bioaugmented SBR.