经济林木离体培养研究进展

Advances on in vitro Culture of Non-timber Forest stock

为了全面了解经济林木离体培养现状和找出离体培养的规律 ,概述了经济林木胚胎培养、器官培养、愈伤组织培养、细胞悬浮培养、原生质体培养、原生质体融合和人工种子等方面的研究现状 .到目前为止 ,已离体培养成功的经济林木种类有 5 0余种 ,其中 ,原生质体培养成功的种类不足 10种 .并指出 ,经济林木愈伤组织再生体系的建立是细胞培养、原生质体培养和原生质体融合的基础 .原生质体培养体系的建立对各种遗传操作和利用生物技术培育新品种具有重要意义 .

In order to overall study the present situation of in vitro culture of non timber forest seedlings and to find out the law of in vitro , this paper summarizes the studying advances on embryo culture, organ culture, callus culture, cell suspension culture, protoplast culture, plasmgamy, and artificial seeding of non timber forest seedlings. Up to now, over 50 seedlings of non timber forest have successfully been cultivated in vitro . Of them, less than 10 species of protoplast are cultivated. This paper also points out that the regeneration system of callus of non timber forest is a base for cell culture, protoplast culture and plasmgamy. The formation of the system of protoplast culture has an important significance in various kinds of genetic operations and in utilizing biological technology to cultivate new varieties.