微寒性药物对大鼠急性心肌缺血的保护作用

Protection Effect of Ramulus Mori against Acute Myocardial Ischemia in Rats

目的:通过结扎冠状动脉左前降支造成大鼠急性心肌缺血模型,观察微寒性药物桑枝提取物抗心肌缺血作用。方法:将大鼠分为4组,结扎冠状动脉左前降支造成急性心肌缺血。阳性药地尔硫组给药剂量为16mg/kg,桑枝提取液剂量为8g生药/kg,连续灌胃给药7天。于冠脉结扎前、结扎即刻及给药后不同时间点监测心电图,标测ST段毫伏数。冠脉结扎3h后处死动物,取心脏行氯化硝基四氮唑蓝染色,测算心肌梗死面积,腹主动脉取血,测定血清MDA含量。结果:桑枝提取物可明显降低冠脉结扎所致大鼠心肌缺血模型心电图ST段抬高幅度,同时缩小心肌梗死面积,MDA值下降。结论:桑枝提取物对大鼠实验性心肌缺血具有一定的保护作用。

Objective：To investigate the protective effect of extraction of Ramulus Mori on experimental acute myocardial ischemia（AMI）rats.Methods：Rats were randomly divided into 4 groups,and the acute myocardial ischemia model was established by ligating left anterior descending branch of coronary artery（LAD）.The dosage of positive medicine was 16mg/kg.The dosage of Ramulus Mori was 8g/kg,i.g,once a day,7days.ECG was monitored before and at different time points after coronary artery ligation.The myocardial ischemia area was detected by NBT stain.MDA level in the serum was detected.Results：For the Ramulus Mori group,the elevated ST segment lowered,and myocardial ischemia area of AMI rats decreased in a different degree,and MDA level in the serum decreased at the same time.Conclusion：Volatile oil of Ramulus Mori has a protective effect against acute myocardial ischemia caused by LAD ligation.