通心络超微粉以及7-硝基吲唑对脑缺血再灌注大鼠海马一氧化氮的影响

Effects of Tongxinluo Ultramicro-Pulverization and 7-Nitroindazole on NO Concentration in Hippocampus of Rats with Transient Cerebral Ischemia and Reperfusion

目的:研究通心络超微粉以及7-硝基吲唑(7-Nitroindazole,7-NI)对脑缺血再灌注大鼠海马一氧化氮(NO)产生的影响。方法:大鼠前脑缺血采用四血管阻断法,实验分为生理盐水组、通心络超微粉组以及7-NI组。选择性NO测定电极测定NO的浓度。结果:通心络超微粉以及7-NI没有影响大鼠的血压和海马的流量,与对照组相比,均显著减少缺血再灌注时海马内NO的产生(均<0.001)。结论:通心络超微粉、7-NI可以通过抑制神经型一氧化氮合酶(nNOS)从而减少NO的产生而起到神经保护的作用。

Objective：To investigate the efficacy of Tongxinluo ultramicro-pulverization and 7-Nitroindazole（7-NI）on nitric oxide（NO）concentration in rat hippocampus after transient cerebral ischemia and reperfusion.Methods：The model of cerebral ischemia was made by 4-vessel occlusion for 10min.NO concentration was measured directly by an NO-selective electrode method.Saline、Tongxinluo ultramicro-pulverization and 7-NI were administered intraperitoneally 20min before the onset of ischemial.Results：Both Tongxinluo and 7-NI did not affect the hippocampal blood flow and mean arterial blood pressure（MABP）,compared with saline group.Tongxinluo and 7-NI significantly reduced the NO production during post-ischemic early reperfusion（P0.001）.Conclusion：Tongxinluo ultramicro-pulverization and 7-Nitroindazole have protective effect on hippocampal neurons against cerebral ischemia reperfusion injury by reducing the activity of neuronal NO synthase（nNOS）or reducing the content of NO in hippocampus of rat.