补肾方对哮喘缓解期小鼠EOS祖细胞分化的干预研究

Experimental Study of Kidney-Supplementing Formula on Differentiation of Eosinophils Progenitor Cells in Paracmasis Stage of Asthmatic Mice

目的:通过观察补肾方对哮喘小鼠外周血中免疫球蛋白IgE、白细胞介素-5(IL-5)、嗜酸细胞活化趋化因子(Eotaxin)的表达、肺组织病理学改变、骨髓悬液中嗜酸粒细胞(EOS)、嗜酸粒细胞祖细胞(CD3+4)、及趋化因子(CD34+/CCR3+)表达的影响,研究补肾方对嗜酸粒细胞祖细胞的干预作用,并探讨其影响EOS分化的可能机制。方法:选健康雌性BALB/c小鼠50只,随机分5组:空白对照组、模型组、醋酸泼尼松组、补肾方组、补肾方+醋酸泼尼松组,于缓解期用药处理,观察哮喘小鼠的一般情况,外周血IgE、IL-5、Eotaxin表达,骨髓悬液EOS、CD3+4、CD3+4/CCR3+细胞的表达,分析补肾方的疗效、治疗机制。结果:3组治疗组小鼠外周血IgE、IL-5、Eotaxin表达均较模型组明显降低,但各治疗组间无差异;3组治疗组小鼠骨髓悬液EOS计数与模型组相比明显减少,但补肾方组较醋酸泼尼松组小鼠骨髓悬液EOS计数增多,两组间有显著性差异;3组治疗组小鼠CD3+4细胞数、CD3+4/CCR3+细胞计数与模型组相比明显降低,而3组治疗组组间无差异。结论:缓解期服用补肾方可减轻哮喘小鼠复发症状;缓解期服用补肾方对哮喘小鼠气道炎症有一定的抑制作用;缓解期联合应用补肾方和醋酸泼尼松可减少醋酸泼尼松的不良反应;缓解期服用补肾方可能降低哮喘小鼠体内炎症因子水平,抑制EOS的趋化、募集,从而减少CD3+4祖细胞向EOS的分化,最终减轻EOS在肺组织局部的浸润。

Objective：By observing the influence of kidney-supplementing formula on the contents of IgE,EOS,IL-5in peripheral blood,pathological changes of lung tissue and the expression of CD＋34 cell and CC chemokine receptor 3 in bone marrow,to study the intervention effect of kidney-supplementing formula on eosinophils progenitor cells and to explore the possible mechanism.Methods：Fifty healthy female BALB/C mice were randomly divided into five groups： control group,model group,prednisone acetate group,kidney-supplementing formula group,kidney-supplementing formula with prednisone acetate group,which would be treated in the paracmasis stage.By observing the general condition of the mice,the contents of IgE,IL-5,Eotaxin in peripheral blood,pathological changes of lung tissue,and the counts of EOS,CD＋34 cell,CD＋34/CCR3＋ cell in bone marrow to analyze the curative effect and mechanism.Result：The contents of IgE,IL-5,Eotaxin in peripheral blood in three groups of treated mice were significantly lower than those models,but the three treatment groups showed no difference;the counts of EOS in bone marrow in three groups of treated mice were significantly reduced compared with those models,but the EOS in the mice bone marrow of kidney-supplementing formula group increased more than prednisone acetate group,which was significant difference between the two groups;the counts of CD＋34 cell,CD＋34/CCR3＋ cell in bone marrow in three groups of treated mice were significantly reduced compared with those models,but there were no differences among the three treatment groups.Conclusions：Kidney-supplementing formula in the paracmasis stage of asthma can mitigate the symptoms of the asthmatic mouse.Kidney-supplementing formula can inhibit the allergic inflammation in the airway of the asthmatic mouse.The adverse reactions of prednisone acetate can be reduced by combining kidney-supplementing formula and prednisone acetate.With the levels of IL-5,Eotaxin and IgE reduced,kidney-supplementing formula may restrain the differentiation and recruitment of EOS so as to alleviate the local infiltration in lung tissue.