高效液相色谱测定广西生鲜乳中的维生素A

Detection of Vitamin A in the Gungxi fresh milk by performance liquid chromatography

为了建立生鲜乳中维生素A的一种快速、准确、灵敏度高的检测方法,样品经皂化后,经石油醚提取,浓缩后采用高效液相色谱法,以Shim-pack VP-ODS柱(4.6 mm×150 mm,5μm),柱温35℃,检测波长325 nm,流动相为甲醇∶水(98∶2,体积比),流速1.0 mL/min的条件进行实验。结果维生素A在0.174～87.2μg/mL范围内线性关系良好,相关系数r=0.9999,平均回收率为98.17%,RSD=1.90%。建立的方法准确、可靠,可以用于生鲜乳中维生素A的含量检测。

To establish a quick, accurate, sensitive method for analyzing Vitamin A illegally added into the fresh milk. The sample was determined after being saponified, abstracted with petroleum ether and concentrated, performance liquid chromatography was used. The column was Shim-pack VP-ODS 4.6 x150 mm, column temperature was 35 ℃ and the mobile phase was methanol：water = 98：2（v：v） with flow rate 1.0 mL/min and the detective wavelength was 325 mn. The linear range of the calibrati on curve of Vitamin A were 0.174-87.2 μg/mL （r=0.9999） .The mean rates of recovery was 98.17% and the relative standard deviations （n=5） was 1.90%. The method is accurate, realiable that can be used to determine Vitamin A in the fresh milk.