松针褐斑病菌毒素对寄主细胞质膜伤害机理的研究

STUDIES ON THE ROLE MACHINISMS OF THE BROWN SPOT NEEDLE BLIGHT FUNGUS TOXIN TO MAKE HOST CELL DAMAGES

用毒素粗提液处理寄主愈伤组织 2d后 ,可于光学显微镜下观察到严重的细胞原生质泡囊化现象 ,这是寄主受到毒素作用后于细胞水平所表现出的伤害症状。经毒素诱导后用ESR仪检测 ,寄主愈伤组织于 2h内即可产生比对照高得多的超氧阴离子自由基 ,继而诱发膜脂脂过氧化反应 ;随着毒素作用时间的延长 ,寄主细胞膜脂脂肪酸组成发生变化 ,总的变化趋势为不饱和脂肪酸含量减少 ,饱和脂肪酸含量增加 ;毒素作用后于 4h以内即可产生大量的MDA ,6h内则可检测到严重的离子渗漏 ,造成膜的破坏 。

After the host callus cells were inoculated with the toxin, the many bubbles in cell plasma were observed in 2 days. The toxin could induce the more O 2 and spur a peroxidation reaction of the lipid in host callus plasmolemma. and then lead to change the lipid acids composition of the plasmolemma. The toxin could also make the quantity of MDA increased in 4h, and the electrolyte leakage in 6h. Finally, the plasmolemma was damaged, and the cells were killed.