BRL37344对3T3-L1脂肪细胞脂肪分解与脂肪因子表达的影响

Effects of BRL37344 on lipolysis metabolism and adipocytokine gene expression in 3T3-L1 adipocytes

目的观察BRL37344对3T3-L1脂肪细胞脂肪分解及瘦素、TNF-αmRNA表达的影响。方法将3T3-L1前脂肪细胞分化成熟后,用0、10-9、10-7mol/L浓度与0、12、24、48 h作用时间的BRL37344进行干预。采用酶法检测甘油释放含量,RT-PCR法检测细胞瘦素、TNF-αmRNA表达。结果 BRL37344可显著增加3T3-L1脂肪细胞的脂肪分解,10-9、10-7mol/L的BRL37344作用48 h后,细胞内瘦素mRNA表达量分别降低38%、97%,TNF-αmRNA表达量分别降低65%、130%,呈剂量依赖性;10-7mol/L的BRL37344作用12、24、48 h后,瘦素mRNA表达量分别降低6%、48%、119%,TNF-αmRNA表达量分别降低10%、66%、158%,呈时间依赖性。结论 BRL37344可促进脂肪细胞的脂质分解,抑制瘦素、TNF-αmRNA表达,其抗肥胖作用与促进脂肪分解,改善瘦素、TNF-α抵抗状态有关。

Objective To investigate the effects of BRL37344 on lipolysis metabolism and leptin, TNF-α gene expres- sion in 3T3-L1 adipocytes, and to explore its anti-obesity mechanism at the celluar level. Methods The 3T3-L1 preadipo- cytes were cultured and differentiated into adipocytes, then incubated with BRL37344 at different concentrations （0, 10-9 mol/L, 10-7 tool/L） and durations （0, 12, 24, 48 h）. Lipolysis was quantified by glycerol released in the medium which was determined by eolorimetric assay. Leptin and TNF-α mRNA expressions were assayed by RT-PCR. Results Lipolysis increased significantly in 3T3-L1 adipocytes after treatment of BRL37344 in a dose- and time-dependent manner. BRL37344 at doses of 10-9 and 10-7mol/L significantly reduced leptin mRNA expression by 34% and 140% , respectively after 48 hours, as well as TNF-α mRNA expression by 65% and 130%, respectively. Leptin mRNA expression reduced by 6% , 48% , 119% , respectively after treatment of 10-7 mol/L BRI37344 for 12, 24, 48 hours, as well as TNF-α mRNA expression by 10%, 66%, 158%, respectively. Conclusion BRL37344 promotes lipolysis and suppresses leptin, TNF-α mRNA expression in 3T3-L1 adipocytes. And its anti-obesity activity of BRL37344 is related with promoting lipolysis, improving resisting statement of leptin, TNF-α.