青藤碱对糖尿病肾病大鼠肾脏MCP-1表达的影响

Effects of snomenine on monocyte chemoattractant protein–1 in diabetic rat kidney

目的观察青藤碱(sinomenine,SN)对糖尿病肾病大鼠肾脏单核细胞趋化因子–1(monocyte chemoattractant protein–1,MCP–1)的影响,探讨青藤碱对糖尿病大鼠肾脏保护作用。方法 45只链脲佐菌素(streptozotocin,STZ)诱导的SD雄性大鼠随机分成3组,分别为正常对照组(N)组、糖尿病组(D)组、糖尿病予以青藤碱治疗组(S)组。检测24h尿蛋白、血肌酐,RT–PCR法检测肾组织中MCP–1mRNA的表达。结果随着病程的延长,D组大鼠24h尿蛋白排泄量、血肌酐、MCP–1mRNA的表达水平均明显升高,与N组比较有显著性差异(P<0.01);S组大鼠24h尿蛋白排泄量及MCP–1mRNA的表达水平与D组比较明显改善(P<0.01),血肌酐及光镜下病理改变有所好转,但差异无显著性意义(P>0.05)。结论青藤碱对糖尿病肾病具有明显防治作用,其机制可能与降低MCP–1的表达有关。

Objective To investigate the therapeutic effect of snomenine to the diabetic rats on expression of renal monocyte chemoattractant protein–1（MCP–1）.Methods 45 healthy Sprague Dawley rats were divided randomly into three groups：normal comtrol（Group N）,diabetic control（Group D） and snomenine treated group（Group S）,To collect the datas of 24-hour urinary protein excretion serum creatinine reverse transcription polymerase chain reaction（RT-PCR） analysis of MCP–1 mRNA expression.Results There were significant differences in 24-hour urinary protein excretion,serum creatinine and expression of MCP–1 mRNA between the Group D and Group N（P0.01）.The 24-hour urinary protein excretion and expression of MCP–1 mRNA of Group S were markedly greater than those of Group D（P0.01）.But there were no significant differences in serum creatinine and athologic change between the Group S and the Group D.Conclusion Snomenine exerts an early renal protective role to diabetic nephropathy,possibly through inhibition of expression of MCP–1.