高效液相色谱法测定明目地黄丸(浓缩丸)中丹皮酚含量

Determination of Paeonol in Mingmu Dihuang Pills (Concentrated Pills) by HPLC

目的建立测定明目地黄丸(浓缩丸)中丹皮酚含量的反相高效液相色谱法。方法采用Agilent ZorbaxSB-C18(250 mm×4.6 mm,5μm)色谱柱;流动相为甲醇-水(48∶52);检测波长为274 nm;流速为1.0 mL.min-1;外标法计算含量。结果丹皮酚在32.06～1 282.40 ng范围内呈良好的线性关系(r=1.000 0),平均回收率96.38%,RSD为0.80%(n=6)。结论该方法前处理简便、准确,灵敏度高,重复性好,可作为该制剂的质量控制方法之一。

Objective To establish a method for the determination of paeonol in mingmu dihuang pills（concentrated pills）by RP-HPLC.Methods The analysis was performed on a Agilent Zorbax SB-C18 column（250 mm×4.6 mm,5 μm） with methanol-water（48：52） as mobile phase,at a flow rate of 1.0 mL·min-1.The detection wavelength was at 274 nm.The content was calculated with external standard.Results The linear range of paeonol was good within 32.06-1 282.40 ng（r=1.000 0）.The average recovery was 96.38%,with RSD as 0.80%（n=6）.Conclusion This method is simple,accurate,sensitive,repeatable and can be used for quality control of the preparation.