新糖脂类化合物抗革兰阳性球菌作用机制研究

Antimicrobial mechanism of novel oligorhamnoside derivatives of dirhamnoside derivative compound 7 and cleistrioside-5 against Gram-positive cocci

目的研究新糖脂化合物二鼠李糖脂衍生化合物7(DDC7)和鼠李糖脂(C5)抗革兰阳性球菌的作用机制。方法分离纯化金黄色葡萄球菌(ATCC29213)的细胞壁肽聚糖。依据已知新化合物对ATCC29213的MIC结果,用肉汤二倍稀释法,观察新化合物DDC7、C5分别加入不同浓度肽聚糖后,对ATCC29213生长的影响。结果新化合物DDC7、C5对ATCC29213的MIC均为16μg·mL-1。当加入肽聚糖浓度≥32μg·mL-1时,可以阻断新化合物DDC7、C5对ATCC29213的抑菌作用。结论新糖脂类化合物抗菌作用部位在革兰阳性球菌细胞壁肽聚糖上。

Objective To study the mechanism of action of the dirhamnoside derivative compound 7（DDC7） and cleistrioside-5（C5） inhibition of Gram-positive cocci.MethodsPeptidoglycan derived from Staphylococcus aureus（ATCC29213）was separated and purified.According to the known of the novel compounds against ATCC29213 MIC results.Anti ATCC29213 activity of novel compounds with different concentrations of peptidoglycan was determined,employing dilution broth method.ResultsThe MIC of novel compounds DDC7,C5 against ATCC29213 was both 16 μg·mL-1.The activity of novel compounds against ATCC29213 were inhibited,when the concentration of added peptidoglycan ≥ 32 μg·mL-1.ConclusionThe novel oligorhamnosides activity against Gram-positive cocci is taking place on the cell wall of the peptidoglycan.