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桔小实蝇抗菌肽Bactrocerin-2的分离纯化与鉴定 被引量：2

Purification and Characterization of Bactrocerin-2 from Oriental Fruit fly Bactrocera dorsalis Hendel
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摘要 采用阳离子交换层析、RP-FPLC及RP-HPLC分离纯化技术,从细菌诱导的桔小实蝇蛹中分离鉴定出桔小实蝇抗菌肽Bactrocerin-1的同系物Bactrocerin-2。结果表明:Bactrocerin-2含有20个氨基酸残基,分子量为2 311.51 u,序列为VTKTWIKVIRGIGKSKIKWA;并且Bactrocerin-2与Bactrocerin-1的相似性极高,氨基酸同源性达到90%;该肽与鞘翅目Coleoptericin C-末端的20个氨基酸也具有较高的相似性。氨基酸组成分析结果表明,该肽是一种疏水、带正电荷的抗菌肽。该研究将有助于对昆虫天然免疫反应的认知,并为设计有效的抗菌分子奠定基础。 Bactrocerin -2 （20 -residues）was purified dorsalis Hendel by cation-exchange chromatography, Molecular mass of Baetroeerin-2 was determined from the immunized pupae of oriental fruit fly Bactrocera RP-FPLC and RP-HPLC. It was an isoform of Bactroeerin-1. as 2 311.51 u by MALDI-TOF-MS. Complete amino acid sequences was VTKTWIKVIRGIGKSKIKWA. It showed very high similarity to C-terminal sequences of Coleoptericin. The composition of amino acid residues revealed that Bactrocerin-2 was a hydrophobic, positively charged peptide.
作者 党向利 田金环 易辉玉 柴一秋
机构地区 浙江省亚热带作物研究所 温州市虫生真菌资源研究与开发重点实验室 暨南大学材料科学与工程系 华南农业大学动物科学学院
出处 《热带作物学报》 CSCD 北大核心 2012年第3期556-561,共6页 Chinese Journal of Tropical Crops
基金 浙江省自然科学基金(No.Y3110597) 广东省自然科学基金(No.032256)
关键词 桔小实蝇 抗菌肽 Bactrocerin-2 同系物 双亲性 Bactrocera dorsalis Antimicrobial peptide Bactrocerin-2 Isoform Amphipathicity
分类号 Q969.456.8 [生物学—昆虫学]
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参考文献20

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二级参考文献28

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