金叶卫矛的茎段培养及试管繁殖

Stem segment Culture and Tube Propagation of Euonymus Fortunei cv.Gold

以金叶卫矛带腋芽的茎段为外植体进行试管繁殖 ,筛选出各培养阶段适宜的培养基分别为 :( 1)腋芽萌生 ,MS +6 BA 2 .0mg/L +NAA 0 .2mg/L ;( 2 )分化及继代 ,MS +6 BA 2 .0mg/L +NAA 0 .5mg/L ;( 3)生根 ,1/ 2MS +IBA 0 .3mg/L +NAA 0 .5mg/L。

Using the stem segment with axillary buds as the explant,the tube propagation of Euonymus fortunei cv.Gold was studied.The results of the experiments suggested that the appropriate media for respective culture stages were:(1)for germination of the axillary buds:MS+6 BA 2.0mg/L+NAA 0.2mg/L,(2)for differentiation and subculture:MS+6 BA 2.0mg/L+NAA 0.5mg/L,(3)for rooting:1/2 MS+IBA 0.3mg/L+NAA 0.5mg/L.