代谢组学方法研究姜黄根茎及块根次生代谢产物表达差异

Metabonomic Study on Secondary Metabolites of Rhizome and Tuberous Root of Curcuma longa L

基于GC-MS的代谢组学分析方法,建立了分析不同产地来源的姜科植物姜黄(Curcuma.LongaL)的根茎(中药姜黄)和块根(中药郁金)的次生代谢产物的方法。对样品的提取方法、气相升温条件和方法学进行了考察,确定采用石油醚索氏提取法对样品进行提取。色谱图中主要化合物的精密度为1.2%～5.7%;重现性为2.18%～6.27%;回归系数R2=0.699～0.972。数据处理采用SIMCA-P软件进行主成分(PCA)分析,通过主成分得分图(Score plot)可以明显区分姜黄的根茎及块根样品,说明在根茎和块根中次生代谢产物的表达存在差异;通过载荷图(Loading)和t检验(t-test)发现了14种表达差异显著的化合物。这些化合物可能是姜黄根茎与块根具有不同药性和临床用途的物质基础。

A metabonomic method of GC-MS was developed to investigate the difference of secondary metabolites in the rhizome and tuberous root of Curcuma longa L.The extraction conditions,temperature program and methodology were optimized.Using the petroleum ether as the extraction solvent and Soxhlet extraction,the method displayed good precision（RSD： 1.2%-5.7%）,repea-tability（RSD： 2.2%-6.3%） and linearity（R2： 0.699-0972）.The analysis data were performed by SIMCA-P software.Scores plot of PCA could successfully divide the rhizome and tuberous root samples into two groups respectively,which illustrate the difference of secondary metabolites between the rhizome and tuberous root.With the loading plot and t-test,the significant difference in two groups of fourteen endogenous metabolites were found,which may be the effective substance for explaining the different usage of the rhizome and tuberous root of Curcuma longa L.in Chinese medicine.