小麦转TaEBP基因株系抗旱特性分析

Analysis of drought resistance of transgenic wheat with TaEREB2 gene under PEG and different irrigation treatments

以2个转TaEBP基因新春9号小麦株系(G9-X1129,G9-X1139)、受体品种新春9号和当地推广品种小偃22为材料,通过PEG-6000人工模拟干旱胁迫处理,调查小麦种子萌发期胚芽鞘、胚根长度和胚根数;采用大田不同水分处理试验,测定转基因小麦不同生育时期的生理生化、形态指标与抗旱指数,以明确其抗旱性。结果表明:(1)在种子萌发期20%(W/V)PEG干旱胁迫条件下,参试材料的胚芽鞘长度、胚根长度和胚根数均比非胁迫时降低,G9-X1129和G9-X1139的降幅较小且均显著高于受体对照新春9号;(2)与受体对照新春9号相比,G9-X1129和G9-X1139具有较高的可溶性蛋白含量和脯氨酸含量;(3)随着干旱胁迫程度的增强参试材料的株高、穗叶距和穗长均有不同程度的降低,但转基因株系株高、穗叶距和穗长降幅显著小于受体对照;(4)G9-X1129和G9-X1139分别比受体对照新春9号增产11.67%和10.19%,其抗旱指数分别为1.18和1.17,两者都属较强抗旱等级,表现出比新春9号更强的抗旱、节水性能;整体上G9-X1129和G9-X1139与小偃22具相对一致的抗旱能力。

Using two transgenic lines G9-X1129 and G9-X1139 with TaEREB gene,receptor parent Xinchun No.9 and local contrast varieties Xiaoyan 22 as materials,we investigated their coleoptile length,radicle length and radicle number of wheat seedlings under drought stress simulated with PEG osmotic solution,and analyzed their physio-biochemical,morphological and drought resistance index under different water treatments at field case.The results showed that compared with normal,their coleoptile length,radicle length and radicle numbers were reduced under drought stress simulated with 20% concentration of PEG-6000 osmotic solution,but the declining range of the receptor parent was greater than that of the transgenic lines;The soluble protein,proline content of G9-X1129 and G9-X1139 were observably higher than that of Xinchun No.9;With the increase of drought stress,the height,space of spike leaf,spike length of materials had different extent of decrease,but the declining range of the transgenic lines was lower than that of the receptor parent.The yield of G9-X1129 and G9-X1139 exceeded the receptor control by 11.67% and 10.19% respectively,and the drought resistance index was 1.18 and 1.17,which belongs to a strong drought-resistant level.It is suggested that the performance of drought resistance and water saving of the transgenic lines G9-X1129 and G9-X1139 were higher than Xinchun No.9,and the same as Xiaoyan 22.