维吾尔族肥胖伴胰岛素抵抗患者内脂素的表达变化及意义

Changes and significance of serum visfatin levels in Uygur obese patients with insulin resistance

目的探讨维吾尔族肥胖(OB)伴胰岛素抵抗(IR)患者血清内脂素的变化及意义。方法根据胰岛素抵抗诊断标准(胰岛素抵抗指数≥2.8),将患者分为肥胖伴胰岛素抵抗组(OB伴IR组)31例,肥胖无胰岛素抵抗组(OB无IR组)26例,另选14例健康体检者为正常对照组。半定量RT-PCR方法检查研究对象网膜和皮下脂肪组织内脂素mRNA的表达水平。对所有受试者采用稳态模式评估法(HOMA)评价胰岛素抵抗(IR),采用ELISA法测定血清内脂素水平,同时进行临床生化参数的检测。结果(1)OB伴IR组血清内脂素水平高于OB无IR组,差异有统计学意义(P<0.05)。(2)血清内脂素与体质量、甘油三酯(TG)呈正相关,与高密度脂蛋白-胆固醇(HDL-C)呈负相关。(3)3组网膜与皮下脂肪组织内脂素mRNA表达水平差异无统计学意义。结论血清内脂素水平的升高可能与维吾尔族肥胖患者的胰岛素抵抗有关。

Objective To investigate the changes and significance of serum visfatin levels in obese patients with insulin resistance. Methods Patients with insulin resistance index ≥2.8 was diagnosed as insulin re-sistance. A total of 71 cases were divided into obese with insulin resistance group （OB with IR） （n=31）, obesity without resistance group （OB without IR） （n=26） and control group （n=14）. The visfatin mR NA expressions in abdominal omental and subcutaneous adipose tissues by semi-quantitative were investi-gated by reverse transcription polymerase chain reaction （RT-PCR）. All subjects were evaluated by the homeostasis model assessment （HOMA） insulin resistance （IR）, and the ELISA method was used for the determination of serum visfatin levels,while the general examination of blood sugar, lipids and other bio- chemical tests. Results （1） The levels of serum visfatin were significantly increased in OB with IR group than OB without IR group （P〈 d0.05）. （2） The level of serum visfatin was positively correlated with weight and triglyeride （TG）, was negatively correlated with high density lipoprotein cholesterol （HDL-C）. （3） There were no significant differences in the abdominal omental and subcutaneous adipose tissue visfatin mRNA ciated with the expression between the three groups. Conclusion The level of serum visfatin may be asso-insulin resistance.