Clara细胞分泌蛋白10基因G38A多态性与5岁以下喘息患儿的相关性

Relationship between Clara Cell Protein 10 Gene G38A Polymorphism and Pathogens of Wheezing in Children under 5 Years Old

目的探讨5岁以下喘息患儿Clara细胞分泌蛋白10(CC10)基因G38A位点多态性与喘息发病机制的关系。方法随机选取于本院就诊的5岁以下反复喘息患儿120例,分为有特应质高危因素的喘息Ⅰ组(n=67)(湿疹45例,父母或父母一方有哮喘病史13例,变应性鼻炎5例,变应性皮炎4例)和无特应质高危因素的喘息Ⅱ组(n=53);对照组为本院外科近期无感染疾病史、择期进行手术的术前患儿(n=55)。采用PCR-限制性片段长度多态性分析对喘息组和对照组患儿CC10 G38A位点基因型频率和等位基因频率进行检测,比较3组间CC10 G38A位点基因型频率和等位基因频率。结果喘息Ⅰ组、喘息Ⅱ组和对照组3种基因型AA、GA、GG分布频率分别为20.9%、44.8%、34.3%,9.4%、32.1%、58.5%、9.1%、31.0%、60.0%;喘息Ⅰ组和喘息Ⅱ组CC10基因G38A位点基因型频率比较差异有统计学意义(P<0.05);喘息Ⅰ组和对照组CC10基因G38A位点基因型频率比较差异亦具有统计学意义(P<0.05)。喘息Ⅰ组、喘息Ⅱ组和对照组38A和38G等位基因频率分别为43.3%、56.7%,25.5%、74.5%,24.5%、75.5%;喘息Ⅰ组和喘息Ⅱ组、喘息Ⅰ组和对照组比较差异均有统计学意义(Pa<0.05)。结论喘息患儿与哮喘存在相同的基因分布频率,发生哮喘的危险性高;对于CC10基因具有A等位基因的喘息患儿应密切关注。

Objective To study the relationship between Clara cell protein 10（CC10） gene G38A polymorphism and the pathogens of wheezing in children under 5 years old. Methods Children under 5 years old with recurrent wheezing had been randomly enrolled. They were divided into 2 groups ： wheezing group I with atopic high risks included 67 cases （ including eczema 45 cases, or either of their parents had a history of asthma risk factors for atopic quality standards 13 cases, atopic rhinitis 5 cases, atopic dermatitis 4 cases） ; and wheezing group II without atopic high risks （n = 53 ）. Children with surgical operation selectly, without recent infectious diseases had been enrolled randomly as control group（ n = 55 ）. Polymerase chain reaction restriction enzyme cutting technology was used to detect CC10 gene G38A allele frequency and genotypic frequency. Results Frequencies of AA, GA and GG genotypes in wheezing group I ,wheezing group II and control group were 20.9% ,44.8% ,34.3% ,9.4% ,32.1% ,58.5% ,9.1% ,31.0% ,60.0% ; there were significant differences in A38, G38 allele frequency between wheezing group I and wheezing group II ,wheezing group I and control group（ Pa 〈 0.05 ）. Frequencies of A, G allele were 43.3% , 56.7% ,25.5% ,74.5%, 24.5%, 75.5% in wheezing group I , wheezing group II and control group. There were significant differences in A38, G38 allele genotypic frequency in wheezing group I and wheezing group II , wheezing group I and control group （ Pa 〈0. 05 ）. Conclu= sions Wheezing children have the same gene frequency to asthmatic children, which the risk of asthma occurrence is high , we should pay more attention to wheezing children with CCIO gene ,allele A genotypic.