油菜双重不育系花蕾中糖和蛋白质代谢特点

METABOLISM OF SACCHARIDE AND PROTEIN IN FLOWER BUDS OF GENIC+CYTOPLASMIC MALE STERILE LINE OF RAPE ( BRASSICA NAPUS L.)

以甘蓝型油菜细胞质不育 (CMS)和细胞核不育 (GMS)为对照 ,对油菜双重不育系 (GCDMS)品系GCDA - 2花蕾中糖和蛋白质的含量进行测定。结果表明 ,GCDA - 2与CMS和GMS相似 ,比其相应的保持系都有较高的糖和较低的蛋白质含量 ,表明花粉败育的同时 ,营养代谢也发生了紊乱。CMS与GMS的差异达极显著水平 :GCDA群体则介于二者之间 ,核不育株GCDA - 2HA与GMS在糖和蛋白质的表达上是一致的 ,而与CMS有一定差异 ;GCDA中质不育株GCDA- 2BA与CMS相似 ,而与GMS的保持系完全不同 ;说明在GCDA中 ,当双隐性核不育基因呈杂合状态时 ,细胞质不育核基因对糖、氮代谢起作用 :当两套不育系统的核基因都起作用时 。

Contents of saccharide and protein in flower buds of GCDA2, a GCDMS (genic+cytoplasmic double male sterility) line of rape (Brassica napus L.) were determined and compared with those of CMS and GMS lines. Similar to the GMS and CMS lines, GCDA2 had higher saccharide content and lower protein content in their flower buds than its maintainer, suggesting that disorder in nutrient metabolism occurred during pollen abortion. Highly significant differences were observed between the CMS line and the GMS line in saccharide and protein contents in their flower buds, and GCDA appeared in between. It is inferred from the study that when the nucleus recessive male sterile genes of GMS in GCDA are homozygous, the nucleus genes of GMS has priority in their expression and when they are heterozygous, the nucleus recessive gene of CMS can be expressed.