反义miRNA-21/rAV-Tumstatin病毒载体对裸鼠膀胱癌生物学行为的影响

The biological behaviour influence of Anti-sense miRNA-21/ rAV-Tumstatin viral vector on nude mice bladder cancer

目的探讨反义miRNA-21/rAV-Tumstatin病毒载体对裸鼠膀胱癌生长及转移的影响。方法通过构建裸鼠原位膀胱癌移植瘤模型并灌注携带基因的腺病毒载体,观察肿瘤生长及盆腔淋巴结转移情况,同时采用免疫组化法检测肿瘤细胞增殖和凋亡指数。结果肿瘤大小:治疗组为(14.60±5.31)mm2,对照组为(58.92±7.16)mm2,两组比较P<0.01;肿瘤细胞增殖率:治疗组为41.30%,对照组94.70%,两组相比P<0.05;凋亡指数治疗组和对照组分别是30.18和9.53,两者比较P<0.05;治疗组5只中无一只发生盆腔淋巴结转移,而对照组3只出现转移(60.0%),两组比较P<0.01。上述各项组间差异均有统计学意义。结论反义miRNA-21/rAV-Tumstatin病毒载体对人膀胱癌裸鼠原位移植瘤具有良好的靶向性,能够通过抑制肿瘤细胞增殖和加速肿瘤细胞凋亡,遏制实验性裸鼠膀胱移植癌模型的生长转移,从而为该载体用于人类膀胱癌的治疗提供了一定的实验依据。

Objective To investigate the influence of anti-sense miRNA-21/ rAV-Tumstatin viral vector on nude mice bladder cancer growth and metastasis.Methods To establish nude mice situ bladder cancer model and pour into adenovirus vector with gene,observe the growth of tumor cell and transfer of pelvic cavity lymphaden,simultaneously use immunohistochemisty detecting proliferation and apoptotic index of tumor cell.Results Tumor size：treatment group（14.60±5.31）mm^2,control group（58.92±7.16）mm^2,two groups compare P〈0.01;proliferation rate of tumor cell：treatment group 41.30%,control group 94.70%,two groups compare P〈0.05;apoptotic index of treatment and control group are 30.18 and 9.53,two groups compare P〈0.05;no one happen pelvic cavity lymphaden metastasis in 5 treatment group,but control group 3 happen metastasis（60.0%）,tow groups compare P〈0.01.The difference of above-mentioned each groups have statistical significance.Conclusion Anti-sense miRNA-21/rAV-Tumstatin viral vector have satisfactory targeting on hominal situ bladder cancer in nude mice model,by inhibiting tumor cell proliferation and accelerating tumor cell apoptosis,to control the growth and metastasis of experimental nude mice bladder cancer cell,thus provide surely experiment basis for this vector using on hominal bladder cancer treatment.