Rh(D)阴性孕妇血液中胎儿微量Rh(D)红细胞与新生儿溶血病发病率的相关性研究

The Correlation Studies on the quantities of the fetal D-positive RBCs in the D-negative pregnancies be quantified by flow cytometer(FCM) and the incidence of hemolytic disease of newborn(HDN)

目的运用流式细胞术产前非侵入性检测Rh(D)血型不合导致新生儿溶血病,探讨该方法在临床的实用性。方法运用已建立的间接标记法(一抗为IgG抗-D,二抗为FITC-抗人IgG(γ),F(ab’)2)染色,采用流式细胞术检测Rh(D)阴性孕妇血液中胎儿微量红细胞。结果流式细胞术与血清学方法检测RhD血型不合导致新生儿溶血病差异不显著。追踪检测Rh(D)阴性孕妇体内胎儿微量Rh(D)红细胞所占比例数据显示,较早进行产前诊断能在一定程度上帮助预防、诊断HDN。结论建立了流式细胞术产前非侵入性检测Rh(D)血型不合导致新生儿溶血病的方法;孕妇在产前常规作流式细胞术检测,为Rh(D)血型不合导致新生儿溶血病提供了一种可靠的诊断依据。

Objective To use the noninvasive method of flow cytometer（FCM） detecting the hemolytic disease of newborn（HDN）due to the mismatching of Rhesus（D） blood group,Exploring the practical of this method.Methods Using the indirect method（IgG anti-D as the first antibody,FITC-anti-IgG F（ab′）2 as the second antibody） to dyeing the cells,and the trace quantities of the fetal D-positive RBCs in the D-negative pregnancies was quantified by flow cytometer（FCM）.Results Difference between the two methods of flow cytometer（FCM） and serological test was not significant.The detected data in the investigation shows that it could be used to prevent hemolytic disease of newborn（HDN）,if the ante-natal detecting be carried out much earlier.Conclusion Establishing the noninvasive method of flow cytometer（FCM） to detect the hemolytic disease of newborn（HDN）.It may be used for diagnosis of hemolytic disease of newborn（HDN）due to the mismatching of Rhesus（D） blood group in ante-natal detecting.