消痔灵体外诱导肝癌细胞HepG_2凋亡及其对P_(53)、nm_(23)-H_1表达影响的研究

The Effect of XZL on Apoptosis of HepG_2 Cells and Expression of P_(53) and nm_(23)-H_1

目的:观察消痔灵注射液(XZL)体外诱导肝癌细胞HepG2凋亡情况,并初步探讨作用机制。方法:用不同浓度(0.5、1、2、4、8、16g/L)XZL体外培养HepG2细胞。采用MTT法检测HepG2细胞抑制率,Western blotting检测相关凋亡蛋白P53和nm23-H1表达变化。结果:(1)XZL能明显抑制HepG2细胞生长,且呈时间和浓度依赖性,(2)XZL组与空白对照组相比,P53蛋白表达增强,差异性显著(P<0.05);XZL组与5-Fu组相比,P53蛋白表达无显著性差异(P>0.05);与共同作用组相比,P53蛋白表达减弱,差异性显著(P<0.05);但nm23-H1蛋白表达均无显著性差异(P>0.05)。结论:XZL可抑制HepG2细胞增殖,并通过增强P53蛋白表达诱导HepG2细胞凋亡。

Objective： To observe the apoptosis of hepatoma carcinoma cell HepG2-in vitro-induced by Xiaozhiling injection（XZL） ,and discuss its mechanism of action. Methods： HepG2 cells were cultured in vitro with different concentrations of XZL（0. 5.1.2.4.8.16g/L）, and the cell inhibition rates were detected by eytotoxlcity test, then Western blotting was used to detect the changes of correlated apoptosis protein P53 and nm23-H1. Results： （1）XZL significantly restrained the growth of HepG2 cells, presenting time and concentration dependent, （2）compared with the control group, the expression of P53 in the XZL group was markedly higher （P〈0. 05）, compared with the 5-Fu group, there are no significant difference in the expression of P53 （P〉0. 05）, compared with the co-action group, the expression of Ps3 in the XZL group significantly lower（P〈0. 05）, but the difference of expression of nm23-H1 between each group were not significant （P〉0.05）. Condusion：XZL can inhibit HepG2 cells multiplication, and induce the apoptosis of HepG2 ceils by increasing the expression of P53.