卵圆卡盾藻对卤虫的急性毒性效应

Acute toxicity of Chattonella ovata on Artemia sinica

研究了卵圆卡盾藻在不同生长阶段和不同密度下对卤虫的急性毒性效应,结果表明:对数生长初期的卵圆卡盾藻藻液的毒性作用最强,卤虫暴露在4.3×104/mL藻液48 h后,死亡率达到80%;对数生长后期卵圆卡盾藻藻液也具有较强的毒性,48 h卤虫死亡率为67%;衰亡期藻液毒性最弱,48 h卤虫死亡率为40%。卵圆卡盾藻的藻液和滤液均对卤虫有致死作用,且前者毒性大于后者。海洋盾藻藻液和滤液的浓度对数与卤虫的死亡机率单位呈线性关系,藻液对卤虫24 h LD50为4.8×104/mL,而滤液对卤虫的24 h LD50为5.6×104/mL。卤虫在卵圆卡盾藻藻液中的暴露时间(48 h内)与死亡机率呈线性相关关系,在藻密度为5.1×104/mL时,卵圆卡盾藻藻液对卤虫的半致死时间(LT50)为38.6 h。

The acute toxicity ofChattonella ovata in different growth phases and concentrations onArtemia Sinica was investigated. The results showed that the maximum toxicity of C. ovata on A. salina appeared at early logarithmic phase. It was found that the death rate of A. sinica reached to 80% in 48 h when exposed to the cell-suspended culture at early logarithmic phase with algae density being 4.5×104/mL, and the lowest toxicity on A. sinica appeared in decline phase with death rate of 40%. The filtered cell-free cultures of C. ovata also showed the toxic to A. sinica, but less toxic than cell-suspended cultures. The probity mortality of A. salina was closely correlated with log dose of cell-suspended and cell-free culture of C. ovata. The 24 h LDso of cell-suspended and cell-free cul- ture of C. ovata was 4.8×104 and 5.6×104/mL, respectively. The probity mortality of A. salina was also correlated with time. LT,, of A. salina with cell density of 5.1×104/mL were 38.6 h.